Dulbecco’s PBS without Ca2+and Mg2+ (D-PBS), Sterile, 1X

Cat. No.: DCP-DPBSWOCAMG1X

Size: 500ml, 1000ml (1 Liter), 10 liter

pH: pH 7.4 | Available other customization of pH, other customization of concentration (2X, 4X, 5X, 10X) | Please Inquire

Storage: Room Temperature; 4°C recommended for a long term storage

FormAppearance: Sterilized Liquid, Clear, Colorless

Components: 2.67mM Potassium Chloride (KCl: 200.00 mg/L), 1.47mM Potassium Phosphate monobasic (KH2PO4 : 200 mg/L), 8.10 mM Sodium Phosphate Dibasic (Na2HPO4: 1150mg/L), and 8.1 mM Sodium Chloride (NaCl, 8000 mg/L) | Available for other customizationaddition of chemicals/detergents/inhibitors/proteins/others | Please Inquire

Sterilization: Prefiltered with 1 µMeter, followed by filtering 0.1µMeter membrane

Dulbecco’s phosphate-buffered saline (DPBS) is a balanced salt solution designed for multiple cell culture applications due to its osmotic balance property similar to the natural condition of mammalian systems. D-PBS is generally used to provide the appropriate milieu to sustain the physiological and structural integrity (i.e. tonicity/viability) of cells in vitro briefly. Balanced Salts support viable conditions for cells only during experimental conditions outside of their regular growth environment. When treated with D-PBS, the cell maintains osmotic balance and physiological pH, and D-PBS without Calcium and Magnesium protects the cells from acute fluctuations.

A wide range of uses include (a) general cell culture, (b) washing adhesion cells before dissociation, (c) transporting cells or tissue samples, (d) diluting cells for counting, and (e) preparing reagents for providing inorganic salts essential for basic cell metabolism. D-PBS is widely used because its buffering capacity is effective at in vivo neutral pH in various applications. When included with calcium and magnesium, trypsinization/ cell dissociation is less effective, but such inclusion is useful for transport media or for reagent preparation. In addition, D-PBS is generally used as a diluent or as an inorganic base for standard media preparation. Deterioration of the D-PBS may be recognized by precipitate or particulate matter throughout the solution, cloudy appearance, color change, and/or pH change.

All DiagnoCine Precision Sterile reagents are filtered-sterilized with a 0.1-micron filtration, thus mycoplasma contamination is largely prevented. The smallest size mycoplasma type can be about 0.2 microns.

Feature:

CaCl2 (-), MgCl2 (-), Protease (-), DNAse (-), RNAse (-), Endonuclease (-)
Used ASTM 0.05 µS/cm Type 1 Ultrapure H2O | Deionized
Tissue CultureMolecular Biology grade

RUO* | R&D use only | Not for human therapeutic use or human consumption
*RUO – Research Use Only

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided ‘AS IS’ and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.