KOD One PCR Master Mix
Product#: KMM-101 // Try Diagnocine free trial sample now
Feature: TOYOBO KOD One High Fidelity PCR Master Mix shows Superior Amplification Performance for 10Kb+, GC-Rich Regions, and Low Expression Genes
Compared to other products in PCR Master Mix (Third-Party experimental data shows)
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(1) Greater than ten times better than ThermoFisher DreamTaq for High GC Content PCR
Do you have a high GC content or touch gene to amplify? KOD One High Fidelity PCR master mix is ten times better than Dream Taq (Thermo Fisher) for High GC Content template (amplification of PIPPA gene region)
No band due to a really tough GC region? KOD One (product #KMM-101 or 201 series) is 10X superior to Dream Taq, generating that band for you from all High GC content regions.
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| Thermo Fisher’s DreamTag | TOYOBO KOD One Mix (KMM-101) |
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• PCR MasterMix (2X) comparison
• Target: Tough GC region of PIPPA gene
• Data provided by Customer from NICHD/NIH
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(2) Greater than ten times better than Invitrogen or ThermoFisher Platinum Taq (High Fidelity Fast PCR)
Wish to generate solid PCR bands? KOD One Master Mix (product #KMM-101 or 201 series) is more than ten times better than Invitrogen | Thermo Fisher Platinum Taq (amplification of XTL1127 region).
KOD One is an extremely effective enzyme that will amplify tough genes and DNA templates. Abundant PCR amplification allows you to load a small amount of PCR product and save PCR samples for later use.
| Invitrogen’s Platinum Taq / TF |  |
| TOYOBO KOD One Mix (KMM-101) |
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(3) Three times better than NEB OneTaq (Expression Analysis by PCR)
Want something better and stronger than NEB One Taq? KOD One PCR master mix (product #KMM-101 or 201 series) is at least three times better than One Taq for amplifying tough templates.
Researchers confirm that 3X superior amplification for A5 and B6 genes and make solid PCR bands. Excellent enzyme to amplify low abundance genes and tough genomic regions.
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| NEB’s OneTaq | TOYOBO KOD One Mix (KMM-101) |
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• PCR performed under NEB protocol
• Data provided by Customers from NIDDK/NIH
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(4) 30% to 46% lower price than NEB Q5
Want to save budget? KOD One PCR master mix (product #KMM-101 or 201 series) will save you 50% compared to NEB Q5. Buy one unit will save near 30%. Buy 5 set bundles and save more than 35% compared to major brands in the market. Buy 10 set bundle and the KOD One mix is near half price compared to other name brands. Price comparison data showed KOD One master mix is the top choice for high fidelity PCR master mix and the biggest bang for the buck in the market.
| Save Money, Save Time, Better Result Price comparison PCR Master Mix – KOD One vs Other companies |
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(5) Five times better GC-Rich Region gDNA PCR result than Takara GXL System or Clontech GXL System
Want to maximize PCR amplification in the tough template region? KOD One PCR master mix (product #KMM-101 or 201 series) amplifies five times better than Takara GXL System. KOD One mix will generate bands from tough templates with 3X superior amplification than Clontech GXL. KOD One mix is excellent for an implication of long amplicon (10Kb+), High GC rich regions, low expressed genes and secondary structures templates.
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| Takara GXL System / Clontech GXL System | TOYOBO KOD One Mix (KMM-101) |
| • P= primer sets, C= water control • Data provided by Customers from the G.W. Univ. |
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(6) More than ten times better amplification from crude samples than other brands
* High efficiency: excellent amplification from crude samples
Cannot generate PCR band from a crude sample and unpurified templates? KOD will do it for you. KOD One PCR master mix (product #KMM-101 or 201 series) is an extremely efficient enzyme that can work on crude and dirty samples. KOD One mix is more than ten times better than other major brands. 10X superiority compared to other major brands and with some optimization, generating PCR band from crude samples can be done without much preparation.
| * Amplification from whole blood | * Amplification from mouse lysate |
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| The amplification from whole blood and that from mouse lysate were compared. KOD One PCR Master Mix amplified the targets accurately which competitor brands did not. |
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(7) 11 kb long range for all organisms with high fidelity (10Kb+ gDNA Long Range PCR)
• 25ul Rx vol., 25-35 cycles, gDNA Long range PCR
Need a long rang PCR? 10kb or more needed with minimum errors? KOD One PCR master mix (product #KMM-101 or 201 series) can provide greater than 10Kb and will satisfy all long-range PCR experiments. Long PCR works from bacteria to humans with difficult regions on DNA templates. KOD One is highly efficient in generating large-size PCR bands with minimal errors (1 error in 10 million base pairs).
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| (I) Data provided by Customer from the NIAID/NIH | (II) Data provided by Customer from NIDDK/NIH |
(8) 80 times higher fidelity than Taq DNA polymerase and Top Class in the market
Want top high fidelity PCR enzyme and robust amplification of high GC content, long PCR, crude samples, minimum errors. Look no further because the KOD One PCR master mix (product #KMM-101 or 201 series) works above and beyond to give you PCR band at the lowest price per PCR reaction. It is the top-class high fidelity PCR enzyme master mix in the market. KOD One is 80 times higher fidelity than traditional Taq DNA polymerase. It is the most robust PCR enzyme engineered to give you the PCR band you need.
| Diagnocine Toyobo | |
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| Nutation Frequency (10-5) The number of misincorporated bases / 100,000 bases |
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| The error ratios of various PCR enzymes were compared for a human β-globin gene. The amplicons were cloned into the vector and sequences were determined. KOD One PCR Master Mix and KOD One PCR Master Mix- Blue- showed excellent fidelity. The mutation frequency was ~80 times lower than Taq DNA polymerase. | |
(9) Ten times faster than traditional PCR enzymes (1kb per one-second extension)
Looking for a lighting fast PCR enzyme? KOD One PCR master mix (product #KMM-101 or 201 series) will amplify ten times faster than the traditional PCR enzyme mix. 1kb elongation per 1 second extension time gives you 10X times faster PCR time to shorten your bench time and quickly see the result. Shorten your PCR time for long-range amplification with minimum error. Superior and fast enzyme for 10kb and more length of amplicons from all sources of samples and crude sample preparations.
| Various targets were amplified with KOD One PCR Master Mix. (1 sec for up to 1kb; 5 sec per kb for 1 to 10kb, and 10 sec per kb for beyond 10kb) |
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(10) More than ten times better amplification with RNase inhibitors.
Need robust PCR enzyme with RNase inhibitors? KOD One PCR master mix (product #KMM-101 or 201 series) is more than ten times better than other major brands at amplifying PCR bands with RNase inhibitors in the reaction. Samples with RNase inhibitors are no problem with the KOD enzyme mix. No band or weak band becomes a solid and bright PCR band on your gel. Minimum errors, high fidelity, large PCR bands and lightning-fast amplification for all sources of samples, including crude samples. The next-generation high fidelity PCR enzyme is engineered to amplify PCR bands from GC-rich regions and tough templates.
| Minimum effect on amplification in presence of RNase inhibitors in the reaction mix | |
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With Diagnocine Toyobo 1 125 ng RNA equiv. M 1kb DNA Ladder Major Competitor Brands |
Inhibitory effect of RNA in cDNA was compared using various PCR enzymes from major brands in the market. KOD One Master Mix was not susceptible to RNA inhibition and can amplify targets with Rnase inhibitors in the reaction. TOYOBO brand KOD One PCR Master Mix can amplify under 1ug of cDNA with RNase inhibitors (*Lane 4 each case). |
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Buy Now >>> Go to Diagnocine shop <<<
KOD One PCR Master Mix
(1) Greater than ten times better than ThermoFisher DreamTaq for High GC Content PCR
(3) Three times better than NEB OneTaq (Expression Analysis by PCR)
(4) 30% to 46% lower price than NEB Q5
(5) Five times better GC-Rich Region gDNA PCR result than Takara GXL System or Clontech GXL System
(6) More than ten times better amplification from crude samples than other brands
(7) 11 kb long range for all organisms with high fidelity (10Kb+ gDNA Long Range PCR)
(8) 80 times higher fidelity than Taq DNA polymerase and Top Class in the market
(9) Ten times faster than traditional PCR enzymes (1kb per one-second extension)
(10) More than ten times better amplification with RNase inhibitors.
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